Journal: RSC Advances

Article Title: Acid-responsive metal organic frameworks with photothermal effects for osteoarthritis multiple therapy

doi: 10.1039/d5ra04833h

Figure Lengend Snippet: Inhibition of the inflammatory factor expression of the IL-1β (10 ng mL −1 )-treated chondrocytes by EA and the EA-MOF (100 mg mL −1 ). (A) Relative mRNA levels of chondrogenic markers (ACAN), anti-inflammatory gene (GSH-px, SOD-1) and OA-relative genes (IL-6, MMP-13, and MMP-3). ( n = 3, mean ± SD, *** p < 0.001). (B) The expression of IL-6 was detected via immunofluorescence staining. (C) The expression of MMP-13 was detected via immunofluorescence staining (scale bar: 100 µm). (D) Quantification of fluorescence after incubation with EA or EA-MOF for 24 h ( n = 3, mean ± SD, and *** p < 0.001).

Article Snippet: Subsequently, 200 μL of primary antibody MMP-3 (1 : 200 dilution, Boster, China) or IL-1 (1 : 200 dilution, Boster, China) was added to the cell slides and incubated at 4 °C overnight in the dark.

Techniques: Inhibition, Expressing, Immunofluorescence, Staining, Fluorescence, Incubation

Journal: Pharmaceuticals

Article Title: Biluo Qianyuan Formula Ameliorates Post-Traumatic Osteoarthritis by Suppressing FN1-Mediated Synovial Inflammation and Restoring Joint Homeostasis

doi: 10.3390/ph19030500

Figure Lengend Snippet: Integrated network pharmacology and molecular docking analysis of candidate compounds and targets. ( A ) A Venn diagram illustrates the intersection of differentially expressed genes from the GSE178557 dataset with disease-related genes from GeneCards and drug-target genes from DrugBank. ( B ) The PPI network of overlapping targets was generated to visualize functional associations. Central nodes (red) indicate core hub genes identified based on degree centrality and connectivity. ( C ) A Sankey diagram correlates specific target genes ( left ) with their respective enriched KEGG biological pathways ( right ). Connectivity indicates involvement in signaling cascades such as the AGE-RAGE, TGF-beta, and IL-17 signaling pathways. ( D ) Representative 3D docking poses demonstrate the binding orientations and intermolecular interactions between active compounds (bavachinin, hederagenin, and myricanone) and primary protein targets (FN1, MMP3, and TGF-β). ( E ) A heatmap displays the molecular docking scores (binding energy, kcal/mol) for the interaction between candidate compounds and hub targets. Color intensity and numerical values represent the predicted binding stability.

Article Snippet: The serum concentrations of MMP3 (Cat# E-EL-M0626, Elabscience Biotechnology Co., Ltd., Wuhan, China), TGF-β (Cat# E-EL-M0051, Elabscience Biotechnology Co., Ltd., Wuhan, China), and FN1 (Cat# KE00039, Elabscience Biotechnology Co., Ltd., Wuhan, China) were quantified by ELISA using commercial kits according to the manufacturers’ instructions.

Techniques: Generated, Functional Assay, Protein-Protein interactions, Binding Assay

Journal: Pharmaceuticals

Article Title: Biluo Qianyuan Formula Ameliorates Post-Traumatic Osteoarthritis by Suppressing FN1-Mediated Synovial Inflammation and Restoring Joint Homeostasis

doi: 10.3390/ph19030500

Figure Lengend Snippet: BLQYF attenuates systemic inflammation and FN1-associated responses in PTOA. ( A ) Serum levels of MMP3, TGF-β, and FN1 in sham, PTOA, and PTOA mice treated with BLQYF or celecoxib. ( B ) Representative morphology and vimentin immunofluorescence staining of primary fibroblast-like synoviocytes (FLSs) isolated from synovial tissues of patients with post-traumatic osteoarthritis. Nuclei were counterstained with DAPI. Scale bar = 20 μm. ( C ) qRT–PCR analysis of MMP3 , TGF-β , and FN1 mRNA expression in FLSs from PTOA patients and mesenchymal stem cells (MSCs) from non-osteoarthritic hip arthroplasty donors. ( D ) CCK-8 assay showing MSC viability following BLQYF treatment (0–80 ng/mL, 24 h). ( E ) qRT–PCR analysis of MMP3 , TGF-β , and FN1 expression in FLSs following FN1 knockdown and BLQYF treatment (5, 10, 20 ng/mL, 24 h). Data were presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests. p < 0.05, p < 0.01, p < 0.001.

Article Snippet: The serum concentrations of MMP3 (Cat# E-EL-M0626, Elabscience Biotechnology Co., Ltd., Wuhan, China), TGF-β (Cat# E-EL-M0051, Elabscience Biotechnology Co., Ltd., Wuhan, China), and FN1 (Cat# KE00039, Elabscience Biotechnology Co., Ltd., Wuhan, China) were quantified by ELISA using commercial kits according to the manufacturers’ instructions.

Techniques: Immunofluorescence, Staining, Isolation, Quantitative RT-PCR, Expressing, CCK-8 Assay, Knockdown

Journal: Oncology Letters

Article Title: Expression and clinical significance of extracellular matrix metalloproteinase inducer, EMMPRIN/CD147, in human osteosarcoma

doi: 10.3892/ol.2012.981

Figure Lengend Snippet: Correlation between EMMPRIN/CD147 expression and clinicopathological characteristics.

Article Snippet: The sections were blocked with 5% bovine serum albumin (Zhongshan, Beijing, China) in PBS solution for 20 min and probed with rabbit anti-human EMMPRIN/CD147 antibodies (1:300; Boster) at 4°C for 12 h. After washing, the bound antibodies were detected with biotinylated goat anti-rabbit IgG (1:100) and SABC complex at 30°C for 20 min.

Techniques: Expressing